Munich, Germany / 04/10/2018 - 04/13/2018
Within the Fraunhofer-funded project “Mavo OptisCell”, the three Fraunhofer Institutes, Fraunhofer Institute for Laser Technology ILT, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB and Fraunhofer Institute for Applied Information Technology FIT, investigate an automatic process chain for single cell selection, to identify high-producer cell lines for the production of biologicals.
The process of cell analysis is done contact- and marker-free by Raman-spectroscopy.
The spectra are being analyzed and classified with numerical algorithms to identify the production properties of proteins of certain cells.
Subsequently, cells with good production properties are transferred contact-free using Laser Induced Forward Transfer (LIFT) into 96-wellplates. In order to cultivate only those cells with high protein production rates, a second Raman-analysis for quantification of protein expression is done.
This allows the saving of time and costs for the early identification of high-producer clones for the production of biologicals within the production chain.
The new LIFTSYS® Workbench Cell Picker enables the optical analysis of cells and their contact-free transfer via Laser Induced Forward Transfer (LIFT) in a closed, air-conditioned workspace.
Due to its small dimensions, the system can be integrated into a laboratory clean bench. All functions can run under program control giving access to fully automated selection and transfer of cells.
Thereby, the time-consuming manual handling of the cells can be reduced and a reproducible process can be established by eliminating person-specific influences.
Fraunhofer ILT is developing material systems and a process technology for the personalized production of (micro-) optics using stereolithography.
This is based on the use of thiol-en click chemistry to polymerize the photo resins, which can be used to produce optics with low optical dispersion and a high refractive index
In addition to its favorable optical properties, Thiol-En klick chemistry also offers advantages due to its immanent biocompatibility and the option of subsequent surface coating with functional groups.